rabbit anti nanog primary antibody  (Cell Signaling Technology Inc)

Journal: Stem cell research

Article Title: An induced pluripotent stem cell line (NCATS-CL9075) from a patient carrying compound heterozygote mutations, p.R390P and p.L318P, in the NGLY1 gene

doi: 10.1016/j.scr.2021.102400

Figure Lengend Snippet: Characterization of NCATS-CL9075 iPSC line. A) Left: Phase contrast imaging of NCATS-CL9075 colonies on Matrigel coated plates at passage 12. Right: Immunofluorescent montage of iPSCs marker expression: SOX2, OCT4, TRA-1–60, NANOG, and SSEA4 with Hoechst 33,342 labelled nucleus (in blue). B) Flow cytometry analysis of pluripotency protein markers: TRA-1–60 and NANOG respectively. C) G-banding analysis showed a normal karyotype (46, XY). D) Detection of heterozygous gene mutations p.L318P p.R390P in the NGLY1 gene. E) RT-PCR verification of SeV and factors clearance from reprogrammed cells using sendai virus vector transduced fibroblasts as a positive control. F) Histopathological analysis of teratoma displaying normal ectodermal, mesodermal and endodermal differentiation.

Article Snippet: Pluripotency Primary Marker , Rabbit anti-NANOG , 1:400 , Cell signaling, Cat# 4903, RRID: AB_10559205.

Techniques: Imaging, Marker, Expressing, Flow Cytometry, Reverse Transcription Polymerase Chain Reaction, Virus, Plasmid Preparation, Positive Control

Journal: Stem cell research

Article Title: An induced pluripotent stem cell line (NCATS-CL9075) from a patient carrying compound heterozygote mutations, p.R390P and p.L318P, in the NGLY1 gene

doi: 10.1016/j.scr.2021.102400

Figure Lengend Snippet: Reagents details.

Article Snippet: Pluripotency Primary Marker , Rabbit anti-NANOG , 1:400 , Cell signaling, Cat# 4903, RRID: AB_10559205.

Techniques: Immunocytochemistry, Marker, Flow Cytometry, Mutagenesis